Purification scales range from 1g to 40 kg/batch final peptide.

Crude peptides are typically purified by reverse-phase High Pressure Liquid Chromatography (RP-HPLC or HPLC). Using microwave peptide synthesis often results in higher purity crude products, making the purification process easier and more efficient.

Additional purification methods employed by AmbioPharm include crystallization, ion exchange chromatography (IEX), which is particularly useful in the case of pegylated peptides for removal of the free, unreacted PEG, and size exclusion chromatography (SEC) may be used to remove high-molecular-weight impurities and polymers such as with multi-disulfide peptides. AmbioPharm employs a wide range of HPLC columns to handle nearly any scale.

HPLC Column Sizes

• 5, 8, 15, 20, 30cm HPLC Column (Up to 7 kg/batch and 100 kg/year)
• 45cm HPLC Column (Up to 15 kg/batch and 120 kg/year)
• 60cm HPLC Column (Up to 28 kg/batch and 200 kg/year)
• 100cm HPLC Column (Up to 40 kg/batch)

After purification, the resulting peptide is dissolved in rather large volumes of acetic acid and water. It is necessary to obtain the final, purified product in powder form. The techniques employed include lyophilization, precipitation, crystallization, and spray-drying. AmbioPharm has the capacity to isolate purified peptides in a wide variety of scales.

Isolation

• 300L Precipitation Reactor
• 50L Rotary Evaporator
• 100L Inverted Centrifuge
• Vacuum Tray Oven Dryer

Lyophilization

• Multiple Manifold Lyophilizers (up to 1kg/batch and 50kg/year)
• 100L Tray Lyophilizers (up to 3kg/batch and 150kg/year)
• 200L Tray Lyophilizers (up to 6 kg/batch and 250kg/year)
• 400L, 500L Tray Lyophilizers (up to 8kg/batch and 400kg/year)
• 800L, 1000L Tray Lyophilizers (up to 15kg/batch and 800kg/year)