It is important to understand the difference between net peptide content and total peptide content. The lyophilized peptide powder shipped to you usually contains not only peptide, but also some other substances such as water, adsorbed solvents, counterions and salts. The total peptide content refers to the weight of this mixture (Gross Weight). Net peptide weight indicates the actual weight of the peptide component of your sample. Net peptide content is usually 60-90% of the total peptide weight (also called gross peptide weight) and is usually determined by elemental analysis, amino acid analysis or UV spectrophotometry. Net peptide content should not be confused with purity. Purity defines the percentage of the target peptide sequence in the peptide component of your sample.
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