How do you introduce the dye when making fluorescently-labeled peptides?

When making fluorescently-labeled peptides, we always suggest that you add a spacer such as Aminoethyloxy-ethyloxy-acetic acid (Aeea) or Amino caproic acid (Aca) between the peptide and the dye molecule. This helps to minimize the possibility that the dye may affect peptide folding and/or binding to its receptors. If a dye is incorporated as an isothiocyanate linkage (fluorescein isothiocyanate, FITC) to an N-terminal residue, it is imperative to use a linker such as β-Ala, Aca or Aeea to avoid Edman Degradation of the dye and first amino acid from the peptide.

Interested in learning more about Peptide Manufacturing? Contact Us.

Go back to all FAQs