How are peptides synthesized?

All peptides are chemically synthesized from the carboxy-terminus (C-terminus) to the amine-terminus (N-terminus) of the sequence. Usually in solid-phase peptide synthesis (SPPS), the C-terminal residue (amino acid located at the rightmost or carboxylic acid end of the peptide) is either preloaded on the solid-phase support (in the case of Wang resins) or loaded directly at the start of synthesis (in the case of 2-CTC (2-chlorotrityl chloride) resins or Amide resins). In some cases, solution phase (or liquid phase) peptide synthesis methods are used or a hybrid of both solid-phase and solution phase methods.

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